Three distinct evidence standards
A government project count measures aggregate F1 production. Known Neocaridina full-sibling offspring measure pedigree structure for behavior. Caridina microsatellite broods measure biological paternity. Commercial color-cross claims generally lack scorable F2 totals.
Research records
Taiwan national F1 breeding program — 3,000+ offspring
More than 3000 across nine groups; NOT nine replicated controlled color-pedigree denominators
Caridina ensifera — microsatellite multiple-paternity family tree
20 broods; 5–30 offspring per brood; mean 15.65 offspring; all 20 had multiple fathers
Neocaridina davidi — 1,191-offspring controlled pedigree
1,191 offspring; 75 full-sib families; 37 paternal half-sibships; F2 deliberately removed
Neocaridina maternal red grade versus hatchling traits
Two broods/female assessed; accessible abstract does not report a complete color-line F1/F2 phenotype denominator
Neocaridina sibling mating versus unrelated mating
First and second brood experiments; source abstract does not provide total hatchlings by grade
Neocaridina maternal feed versus egg carotenoids
Three diet arms; 40%/52% egg-carotenoid differences, NOT color-allele segregation percentages
Neocaridina denticulata — red, yellow, blue molecular pigment candidates
Three biological replicates for qPCR and pteridine measurements; not F1/F2 parentage cohorts
Neocaridina crustacyanin function — 24 reported homologs
24 CRCN gene models reported under study pipeline; NOT 24 commercial color genes
NdCRCN-30 RNAi coloration and cuticle study
13 CRCN homologs identified in this study; assay NOT an F1/F2 cross
Blue Jelly × Red Sakura — Rili breeder observation
Author suggests 100% F1 red Rili; no sample count, stock IDs or scored family data given
Blue Jelly × Orange Sakura — 25% Orange Rili breeder estimate
About 25% Orange Rili asserted in secondary expert summary; no identifiable brood n
Blue Jelly × Blue Dream — unpredictable F1/F2 mixtures
Qualitative F1/F2; no reliable observed denominators
2007 Cherry × wild-type 'experiment' — protocol only
Zero verifiable outcomes; no genotype tested
Neocaridina red/brown/white lines — substrate color and pigment
Multiple morphs studied; no parent-pair F1/F2 cross cohort reported