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Caridina ensifera — microsatellite multiple-paternity family tree

Caridina ensifera · Controlled genetic parentage assay of natural broods

Species, evidence class and date

Caridina ensifera · Controlled genetic parentage assay of natural broods · 2010

20 berried wild females with 5–30 embryos/hatchlings per brood; four variable microsatellite loci, GERUD and COLONY inference then Multiple sires in each of 20 sampled broods; GERUD identified 58 putative fathers over broods, COLONY inferred 105 sire contributions over broods then 20 broods; 5–30 offspring per brood; mean 15.65 offspring; all 20 had multiple fathersSOURCE / PARENT DESIGN20 berried wild females with 5–30embryos/hatchlings per brood; fourvariable microsatellite loci, GERUDand COLONY inferenceRECORDED INTERMEDIATEMultiple sires in each of 20 sampledbroods; GERUD identified 58putative fathers over broods,COLONY inferred 105 sirecontributions over broodsRESULT / CLAIM20 broods; 5–30 offspring perbrood; mean 15.65 offspring; all 20had multiple fathers
Solid = source-described measurement or study design. Evidence flow; not a color-strain genotype tree.

Parents or study design

20 berried wild females with 5–30 embryos/hatchlings per brood; four variable microsatellite loci, GERUD and COLONY inference

Recorded results

Multiple sires in each of 20 sampled broods; GERUD identified 58 putative fathers over broods, COLONY inferred 105 sire contributions over broods

Numerical counts and denominator

20 broods; 5–30 offspring per brood; mean 15.65 offspring; all 20 had multiple fathers

Claims requiring caution

None

Important limitations

Wild Sulawesi Caridina ensifera, not Taiwan Bee; each inferred sire count is a model output, not independently observed fathers. It establishes parentage complexity, not designer strain color dominance.

Claim-level source ledger